Bacterial Bioluminescent Biosensors
Bioluminescence is a visible light emitted from a living organism, such as the glow of a firefly or jellyfish. The bioengineered bacteria used as bioremedial treatment agents are single-celled organisms that don't glow naturally, but are modified by scientists to do so. The altered bacteria each carry a reporter protein and a sensor.
Let's start with the importance of the reporter protein. Bioluminescence is a chemical reaction caused by an enzyme-catalyzed process: The enzymes that produce light are called luciferin, and those that are the catalyst are called luciferase. The reporter is responsible for this enzymatic activity, which releases bioluminescence energy and gives the bacteria its color. You won't see this bacteria's blue-green glow in the ocean though; its reporter protein is visible under a microscope or with a special light recording device.
The sensor is how the bacteria is able to continually check for the presence of certain chemicals, determined by the scientists who bioengineer the organisms. It also allows the bacteria to detect the concentration and distribution of pollutants and whether or not they are spreading.
Bioremedial treatments are a relatively cheap and easy recovery method compared to traditional chemicals and equipment. In initial studies, bacteria has shown its ability to clean up oil spills more rapidly than traditional techniques and reduce damage to ecosystems in the process.
Environments rich in phosphorous and nitrogen (water-soluble fertilizers found in natural elements like soya bean lecithin and bird excrement) encourage the bacteria to thrive, eating toxic waste and leaving carbon dioxide and water as the only byproducts of their consumption. In such an environment, bioremedial treatments have been found to remove 87 percent of oil within 18 days [source: European Commission, Environmental DG]. When the food (in this case, hydrocarbons) runs out, the bioengineered bacteria die.
Although the bacteria are reported to be safe for release in the natural environment due to their inability to reproduce well in the open and their natural death cycle, government regulations often make it difficult to use them because they have been genetically altered. For now, the bacterial agents used to gobble up oil spills tend to be naturally occurring types with a taste for toxic waste.
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- Allard, Simon T.M. "Bioluminescent Reporter Genes." Promega Corporation. 2008. http://www.promega.com/enotes/features/fe0030.htm
- Andersen, Jens Bo, Arne Heydorn, Arne, Morten Hentzer, Leo Eberl, Otto Geisenberger, Bjarke Bak Christensen, So/ren Molin, Michael Givskov. "gfp-Based N-Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication." Applied and Environmental Microbiology. 2001http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92623
- Attila, K., K. Howe, T.B. Kirkpatrick, R.W. Wills, R. Hartford-Bailey, M.L. Lawrence. "Development of Bioluminescent Salmonella Strains for Use in Food Safety." BioMed Central-Microbiology. Volume 8. 2008.http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2257966
- Browne, Malcolm W. "Researchers Enlist Bacteria To Do Battle With Oil Spill." The New York Times. 1989. http://query.nytimes.com/gst/fullpage.html?res=950DEFDD1E3BF930A15756C0A96F948260
- "Color-coded Bacteria Can Spot Oil Spills Or Leaky Pipes And Storage Tanks." ScienceDaily. 2008. http://www.sciencedaily.com/releases/2008/09/080910210529.htm
- "Encouraging bacteria to clean up oil spills." European Commission, Environment DG. EnvironmentalExpert.com. 2008. http://water.environmental-expert.com/resultEachPressRelease.aspx ?cid=8819&codi=41125&idproducttype=8
- Etkin, Dagmar Schmidt. "Estimating Cleanup Costs For Oil Spills." Oil Spill Intelligence Report. Cutter Information Corp. 1999. http://www.environmental-research.com/erc_papers/ERC_paper_1.pdf
- Gartner, John. "Oil Eaters Slurp Up Spills." Wired. 2004.http://www.wired.com/science/discoveries/news/2004/12/66017
- "How do you clean up an oil spill?" University of Delaware Sea Grant. University of Delaware. 2004. http://www.ocean.udel.edu/oilspill/cleanup.html
- Meighen, Edward A. "Bioluminescence." Chemistry Explained: Foundations and Applications. http://www.chemistryexplained.com/Ar-Bo/Bioluminescence.html
- "Oil Spill Response Techniques." Emergency Management, U.S. Environmental Protection Agency. http://www.epa.gov/oem/content/learning/oiltech.htm
- "Oil Tanker Spill Statistics: 2007." The International Tanker Owners Pollution Federation Ltd. 2008. http://www.itopf.com/information-services/data-and-statistics/statistics /documents/stats07_000.pdf
- Patin, Stanislav. "Oil Spill." The Encyclopedia of Earth. 2008.http://www.eoearth.org/article/Oil_spill
- Williams, Andrew. "Scientists Develop Oil Spill and Pollution Spotting Bacteria." CleanTechnica. 2008. http://cleantechnica.com/2008/10/01/scientists-develop-oil-spill-and-pollution- spotting-bacteria/