Sewing DNA for Science
It may not happen on a Singer sewing machine complete with foot pedal, but scientists often "sew" pieces of one organism's DNA into another's. The result is called recombinant or "chimeric" DNA, named after chimeras, the mythical creatures that are part lion, part goat and part snake.
Often scientists will insert human DNA into bacterial or yeast DNA [source: Tamarin]. With a little extra engineering, bacteria and yeast can take up the recombined DNA and follow the instructions as if nothing happened. The organisms then make human proteins. The process has many applications in research, industry and medicine. Right now, bacteria and yeast are making huge amounts of human insulin, which is used to treat diabetics [sources: Cold Spring Harbor National Laboratory, Eli Lilly].
In addition to sewing DNA, scientists are also straightening it. Our DNA is coiled, coiled, coiled. To study it, you need to straighten it. One popular way is to attach a bead to either end of the DNA, pick up the beads with a laser beam and gently pull the beads apart, says Patrick Doyle, a chemical engineering professor at MIT.
What in the world do scientists do with straightened DNA? In How Epigenetics Works, you'll learn that the outside world, and even the world of our parents, can influence which of the instructions in our genes our body follows. The environment can "talk" to our cells through molecules that direct the reading of our DNA. By straightening DNA, or at least uncoiling it a bit, scientists can study these modifications. They might watch proteins attach chemicals to our DNA or turn genes on and off. Another use of the bead trick is testing whether drugs meant to bind to DNA will work. Scientists can sense whether the drug has bound to DNA by measuring changes in the tension of the coil [source: Doyle].
If what you want is machines, yes -- researchers are building small devices that don't sew but do straighten DNA. Doyle is making one the size of a postage stamp that sends DNA in a stream of liquid through a funnel, straightening it. It could become part of an environmental sensor that sucks in organisms from the air and detects dangerous microbes by their DNA sequence. Would you like to put Doyle's device in your basement, beside your sewing machine? Not so fast: It's not for sale, and it costs more than $10,000 to make.
But the device that wins the prize for somewhat resembling a DNA sewing machine lives in the labs at Kyoto University. A little larger than a credit card, it also uses liquid to push DNA around on a chip. In a 2008 paper published in the journal Lab on a Chip, the researchers showed they could unfurl a wad of yeast chromosomes and, using flowing liquid and a little hook, peel them apart and stick them to posts. Then, letting the chromosomes wad up again, they wound them around two spools [source: Terao]. The hooks and spools measure in the millionths of a meter -- thousands could fit on the head of a pin. While the device hasn't been tested on human DNA, Doyle says the technical display of manhandling long, easily breakable DNA without breaking it was "pretty cool." "Theirs was a clever way of grabbing any old big strand of DNA and moving it around," he says.
So you can't stitch DNA together with a conventional sewing machine, but scientists can manipulate DNA for our benefit. Keep reading to see what else scientists are up to in the field of genetics.
Related HowStuffWorks Articles
More Great Links
- Animation on recombining DNA from DNA Interactive. (Click on techniques, cutting & pasting, and recombining DNA.)
- Story on how human insulin was made by microorganisms for the first time. (Click on production.)
- Becker, Wayne et al. "The World of the Cell." Benjamin Cummings. 2003.
- Boal, David. "Mechanics of the Cell." Cambridge University Press. 2002.
- Campbell, Neil et al. "Biology." Benjamin Cummings. 1999.
- Cold Spring Harbor National Laboratory. DNA Interactive. "Putting it together: synthetic insulin was made using recombinant DNA. 2003. (10/15/2008) http://www.dnai.org/b/index.html
- Doyle, Patrick. Personal interview. Conducted 10/10/2008.
- Eli Lilly and Company. "Humulin R Information for the Patient." 2008. (10/16/2008) http://pi.lilly.com/us/humulin-r-ppi.pdf
- Lu, Ponzy. Personal interview. Conducted 10/10/2008.
- Tamarin, Robert. "Principles of Genetics." McGraw-Hill. 2002.
- Terao, Kyohei et al. "Extending chromosomal DNA in microstructures using electroosmotic flow." Journal of Physics: condensed matter. No. 18. May 10, 2006.
- Terao, Kyohei et al. "On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures." Lab Chip. Vol. 8, no. 8. August 2008.
- Schmetz Needle Corporation. "Sales Guide Ready Reference: Schmetz sewing machine needles." 2008. (10/15/2008) http://www.schmetzneedles.com/Schmetz_Sales_Guide.pdf