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How Light Microscopes Work

The Basics

Diagram of a typical student light microscope, showing the parts and the light path

A light microscope works very much like a refracting telescope, but with some minor differences. Let's briefly review how a telescope works.

A telescope must gather large amounts of light from a dim, distant object; therefore, it needs a large objective lens to gather as much light as possible and bring it to a bright focus. Because the objective lens is large, it brings the image of the object to a focus at some distance away, which is why telescopes are much longer than microscopes. The eyepiece of the telescope then magnifies that image as it brings it to your eye.

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In contrast to a telescope, a microscope must gather light from a tiny area of a thin, well-illuminated specimen that is close-by. So the microscope does not need a large objective lens. Instead, the objective lens of a microscope is small and spherical, which means that it has a much shorter focal length on either side. It brings the image of the object into focus at a short distance within the microscope's tube. The image is then magnified by a second lens, called an ocular lens or eyepiece, as it is brought to your eye.

The other major difference between a telescope and a microscope is that a microscope has a light source and a condenser. The condenser is a lens system that focuses the light from the source onto a tiny, bright spot of the specimen, which is the same area that the objective lens examines.

Also unlike a telescope, which has a fixed objective lens and interchangeable eyepieces, microscopes typically have interchangeable objective lenses and fixed eyepieces. By changing the objective lenses (going from relatively flat, low-magnification objectives to rounder, high-magnification objectives), a microscope can bring increasingly smaller areas into view -- light gathering is not the primary task of a microscope's objective lens, as it is a telescope's.

We'll take a detailed look at the parts of a microscope later in the article.